The Chair of Biotechnology has just acquired a RNX2 1000 Raman spectrometer. This spectrometer with an excitation wavelength of 993 nm is the first model produced by Kaiser Optical Systems in multi-channel allowing to follow up to 4 bioreactors simultaneously. This spectrometer allows to follow a bioreactor in-situ and in real time while avoiding the parasitic fluorescence typical of biological matrices.

In collaboration with LMOPS, the Chair of Biotechnology of CentraleSupélec is working on the instrumentation of bioprocesses in order to allow in-situ and real-time monitoring to better control the process, to acquire more reliable data and to use this data for retrocontrol.
Based on the Raman effect, the device acquires chemical signatures specific to the molecules constituting the medium. A preliminary correlation step using off-line analysis enables the construction of a chemometric model that is then applied to subsequent cultures without requiring additional sampling. The spectrometer acquires spectra every 10 seconds to 10 minutes without the need for operator intervention and without the risk of damaging the integrity of the culture.

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